Equipment
Workstation
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Z800 64bit 12GB RAM – DLD
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Zen 2012 Desktop license
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ZEN Deconvolution
Zeiss LSM 780 NLO Confocal Microscope System
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Axio Observer.Z1
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Inverted microscope
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Motorized Scanning Stage
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Triggering Hardware (user interface box)
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Polarizers for anisotropy
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X-Cite 120 PC
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Point scanning confocal imaging
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Multiphoton imaging
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Lasers
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Tunable Ti-Sapphire Coherent Chameleon Vision II laser
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405 nm Diode
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Argon (458, 488, 514 nm)
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561 nm
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633 nm HeNe
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Detectors
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32 channel spectral array GaAsP detector,
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Also photon counting,
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Fluorescence Correlation Spectroscopy (FCS)
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plus two high sensitivity PMTs
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BiG NDD (non-descanned) detectors
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Transmitted light detector PMT
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BiG filter sets Blue-Green and Green-Red
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Incubation
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Above room temperature and CO2 control for live cell timelapse
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Y module and Temperature Module S: Cooling ( to below 0C) and heating (up to 90C?) two circulating bath incubation without CO2 control
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Definite focus for NLO
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Fluorescence Cubes Filter Sets
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FL Filter Set 49 DAPI, Ex G365, FT 395, Ex BP 445/50
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FL Filter Set 43 CY 3, Ex BP 545/25, FT 570, Em BP 605/70
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FL Filter Set 38 HE GFP, Ex BP 470/40, FT 495, Em BP 525/50
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FL Filter Set 50 Cy5, EX BP 640/30, FT 660, Em BP 690/50FL
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Filter Set 46 YFP, Ex BP 500/20, FT 515, Em BP 535/30
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FL Filter Set 47 CFP, Ex BP 436/20, FT 455, Em BP 480/40
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Objectives with DIC sliders
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Plan-Apochromat 10x/0.45 WD=2.0 M27
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Plan-Apochromat 20x/0.8 WD=0.55 M27
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C-Apochromat 40x/1.2 W Korr. FCS
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Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
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Plan-Apochromat 63x/1.40 Oil DIC M27
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Zen 2011 Software
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FCS GAaSP
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FRET Hardware
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FRAP Analysis
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ZEN 2011 Exp.Designer/MT/Tiles & Pos Pkg
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ZEN Module 3D VisArt