Contact person for this service is Katie Carter.
- Contaminants such as high salt, poor DNA or PCR stutter can reduce data quality.
- Samples should be properly diluted before submitting
- TRFLPs and similar samples should be column purified before submitting
- Column cleaning can be done by ordering through the CGRB Web Ordering Site
- It is important that you empirically determine what DNA concentrations are optimal for your project
- When starting a new project, we suggest doing dilutions series test (i.e. four samples, each diluted 1:1, 1:5, 1:25, 1:100)
- Consequences: if your samples are too concentrated, it may fail to analyze and/or the data will not be precise. High signal in one color can lead to spectral overlap into other colors, possibly leading to confusing results.
- It is critical that your samples do not adversely affect other researchers’ data. Samples with peak heights exceeding the spectral range of the instrument may interfere with the following injection.
- Optimal peak height is between 500-3000 RFUs
- Evaluate which size standard is appropriate for your samples. It should be used across an entire experiment for accurate cross comparisons.
Dye Sets Available
- D: FAM (blue), HEX (green), NED (yellow), ROX (red, standard)
- G5: FAM (blue), VIC/HEX (green), NED (yellow), PET (red), LIZ (orange, standard)
- PET and NED are only available through ABI
Cost of standards included in the price
- Available standards:
- GS500 ROX (35-500 bp)
- GS500 LIZ (35-500 bp)
- GS400 ROX (50-400 bp)
- GS1200 LIZ (50-1200 bp)
- MapMarker ROX (35-1000 bp)
- GeneScan 120 LIZ (15-120 bp)