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GBS services are only for OSU internal projects. Contact Brett Tyler if you have questions about this policy.
Samples provided in plate format.
Two files to upload with your order: Sample ID sheet (as described under Sample Quantity below) and gel image (as described under Sample Quality below).
GBS: The quantity needed for a GBS experiment is 250ng/sample normalized across all samples to the same concentration (no less than 25ng/ul and no more than 100 ng/ul). Sample ID sheet includes the Sample name associated with plate location, the concentration and volume submitted and any additional pertinent information about the sample.
Determining adapter concentration: If the adapter titration value has not been determined then an optimization step must precede the GBS experiment. One sample at 2 micrograms (ug) of high quality gDNA is needed for the Titration / Optimization experiment.
We require a gel image showing all samples uncut and a subset of these samples (10%) which have undergone restriction digest using an enzyme which is NOT methylation sensitive (i.e. EcoRI, HindIII). A 1% agarose gel is often sufficient. The Λ HindIII digest (ladder) is required.
DNA quality is critical for success in a GBS experiment. Uncut genomic DNA should have a tight band greater than 10Kb while restricted samples should exhibit a smear running from 0.2-10KB.