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GBS services are only for OSU internal projects. Contact Brett Tyler if you have questions about this policy.
Genotyping by Sequencing (GBS) is a technique used for creating a reduced representation library from genomic DNA for the purpose of discovering and/or scoring variants such as SNPs. This method was first described in Elshire et.al. High quality genomic DNA is restricted with a restriction enzyme and barcoded adapters are ligated onto the fragments, one per sample. Both ends of the fragment must have the cut site for the process to work.
The adapters currently available for the CGRB service match the restriction enzyme ApeKI which is a rare cutter. Genomic DNA submitted for GBS library construction must be high quality (high molecular weight with no degradation). This is to ensure both ends the long restriction fragments generated in the library construction have the cut site present for adapter ligation. Additionally the gDNA must be free of inhibitors to restriction digest.
Barcoded samples are then pooled and the library is sequenced. Data output is processed through the TASSEL pipeline for downstream analyses.
A good review article comparing the GBS technique with RAD was published by Davey et. al.
Contact Aaron Trippe 541-737-3413 to discuss your project and sample submissions.
Contact Andrew Black with questions concerning the start of a GBS project, experimental design, and the options for data processing and downstream analysis using TASSEL and other software pipelines.
Contact Matthew Peterson with any questions and for details concerning the processing of your run, e.g., creating the Key file and formatting your reference genome.