The Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System is a technology that provides high-precision with absolute quantification of nucleic acid targets.  Droplet Digital PCR (ddPCR) affords scientists the ability to quantify template molecules that may be undetectable using traditional techniques.  Researchers are utilizing ddPCR to quantify genomic alterations, such as copy number variation, detect rare sequences, and determine absolute quantification of target DNA copies without the need for a standard curve.

Bio-Rad has more information available:

  • Overview page
  • Webinars
  • Applications
    • Liquid Biopsy
    • Copy Number Variation (CNV)
    • Rare Sequence Detection
    • Gene Expression
    • Single-Cell Analysis
    • Pathogen Detection and Microbiome Analysis
    • Next Generation Sequencing (NGS)

The system includes an Automated Droplet Generator (ADG) that generates 20,000 droplets from one ddPCR reaction containing your target or background DNA. Samples are processed 8 at a time. Hydrolysis probes (Taqman) or EvaGreen (SYBR-like) chemistry can be used on the system.  After PCR the samples are transferred to a QX 200 Droplet Reader where individual droplet intensities are read.

The Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System is located in ALS 3012. The system can be operated as a Multi-user instrument, or Full service with sample dropoff for CGRB to run. Run times need to be coordinated with CGRB Core staff during lab open hours.

Service maintained by Anne-Marie Girard and Jessica Nixon.